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NCYC 497

Wickerhamomyces canadensis

Pre 2011 Name Pichia canadensis

Equivalent Strain Designations ATCC 18079, CBS 1992, CCRC 20482, CCRC 22407, DBVPG 6262, IFO 0973, JCM 3597, NRRL Y-1888, UCD 74-71

Depositor L.J. Wickerham, NRRL.

Deposit Date April 1957

Habitat Insect frass on Pinus resinosa (red pine), Ontario, Canada.

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Wickerhamomyces canadensis

Aerobic Utilisation and Growth

Glucose +
Galactose -
Sorbose -
Sucrose +
Maltose +
Cellobiose Unknown
Trehalose +
Lactose -
Melibiose Unknown
Raffinose +
Melizitose +
Inulin -
Soluble Starch -
Xylose Unknown
L Arabinose -
D Arabinose -
Ribose Unknown
Rhamnose +
Ethanol Unknown
Glycerol Unknown
Erythritol -
Ribitol -
Galactitol Unknown
Mannitol Unknown
Sorbitol Unknown
A M D Glucoside +
Salicin Unknown
Lactic Acid Unknown
Succinic Acid Unknown
Citric Acid Unknown
Inositol Unknown
Gluconolactone Unknown
Glucosamine Unknown
Methanol Unknown
Xylitol Unknown

Strain Information

Type Strain
Information Produces lipases active at pH 9.0 at 60C (Hou CT, 1994).
Depositor L.J. Wickerham, NRRL.
Deposit Name Hansenula canadensis
Month of deposit April
Deposit Year 1957
Habitat Insect frass on Pinus resinosa (red pine), Ontario, Canada.
Equivalent Strain Designations ATCC 18079, CBS 1992, CCRC 20482, CCRC 22407, DBVPG 6262, IFO 0973, JCM 3597, NRRL Y-1888, UCD 74-71
Reference Wickerham, US.Dep.Agric.Tech.Bull.1029, Washington 1951, p.26.
Kurtzman, Mycologia 79: 410-417, 1987 (DNA).
Fuson et al., Int.J.Syst.Bacteriol. 29: 64-69, 1979 (DNA).
Yamada et al., J.Gen.Appl.Microbiol. 19: 189-208, 1973 (CoQ.
Hou CT. pH dependence and thermostability of lipases from cultures from the ARS Culture Collection. J. Ind. Microbiol. 13: 242-248, 1994.

Physical Characteristics

Optimum Temperature Unknown
Miniumum Temperature Unknown
Maximum Temperature Unknown

Cells

Shape Oval
Min Broth Breadth (µm) 2
Max Broth Breadth (µm) 3
Min Broth Length (µm) 2
Max Broth Length (µm) 5
Min Agar Breadth (µm) 1
Max Agar Breadth (µm) 3
Min Agar Length (µm) 2
Max Agar Length (µm) 5
Arrangement Pairs
Colour on Agar White
Surface on Agar Shiny
Texture on Agar Smooth
Deposit in Broth Non-Flocculent
Ring in Broth Complete
Ring Colour White
Pellicle in Broth Thin
Pellicle Appearance Milky
Pellicle Habit Climbing

Cell Division

Budding Multipolar
Fission Absent

Filamentous Growth

Pseudomycelium Well formed
Pseudomycelium Branch Unknown
Pseudomycelium Form Mycotorula
Blastospores Many
Blastospore Shape Oval
Blastospore Location Verticils
Blastospore Habit Unknown
True Mycelium Absent
Clamp Connections Absent

Asexual Spores

Ballistospores Absent
Arthrospores Absent
Endospores Absent
Chlamydospores Absent

Sexual Spores

Ascospores Present
Ascospore Shape Hat
Ascospore Wall Smooth
Ascospore No Per Ascus 2
Ascus Shape Unknown
Conjugation Absent
Teliospores Absent
Teliospore Shape Unknown

Miscellaneous

Assay Unknown
Salt Tolerant -
Killer Unknown
Plasmid No

Semi-Anaerobic Fermentation

Glucose +
Galactose Weak/Latent
Sucrose +
Maltose +
Cellobiose -
Trehalose -
Lactose -
Melibiose -
Raffinose +
Melizitose -
Inulin -
Soluble Starch -
Xylose Unknown
A M D Glucoside -

Aerobic Utilisation and Growth - Sole Sources of Nitrogen

NH4 2SO4 Unknown
KNO3 +
Ethylamine Unknown
Cadaverine Unknown
Lysine Unknown

Other

Vitamin Free Growth -
Cyclohex 100ppm Unknown
Cyclohex 1000ppm Unknown
50% Glucose Growth Unknown
60% Glucose Growth Unknown
Lipolytic -
Acid Production Unknown
37c Growth Unknown
40c Growth Unknown
Arbutin Hydrolysis Weak/Latent
Urease Activity Unknown
Starch Production -
Acid Tolerant Unknown