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NCYC 494

Wickerhamomyces bisporus

Pre 2011 Name Pichia bispora

Equivalent Strain Designations ATCC 10627, CBS 1890, CCRC 20468, IFO 0803, JCM 3590, NRRL Y-1482.

Depositor L.J. Wickerham, NRRL.

Deposit Date April 1957

Habitat Tunnel of Ips typographus (bark beetle), under bark of spruce, Austria.

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Wickerhamomyces bisporus

Aerobic Utilisation and Growth

Glucose +
Galactose -
Sorbose Unknown
Sucrose +
Maltose +
Cellobiose Unknown
Trehalose Weak/Latent
Lactose -
Melibiose Unknown
Raffinose -
Melizitose +
Inulin Unknown
Soluble Starch Unknown
Xylose Weak/Latent
L Arabinose Unknown
D Arabinose Unknown
Ribose Unknown
Rhamnose Unknown
Ethanol Unknown
Glycerol Unknown
Erythritol -
Ribitol -
Galactitol Unknown
Mannitol Unknown
Sorbitol Unknown
A M D Glucoside Unknown
Salicin Unknown
Lactic Acid Unknown
Succinic Acid Unknown
Citric Acid Unknown
Inositol Unknown
Gluconolactone Unknown
Glucosamine Unknown
Methanol Unknown
Xylitol Unknown

Strain Information

Type Strain
Information Produces lipases active at pH 5.5 and 7.5 (Hou CT, 1994).
Depositor L.J. Wickerham, NRRL.
Deposit Name Endomycopsis bispora
Month of deposit April
Deposit Year 1957
Habitat Tunnel of Ips typographus (bark beetle), under bark of spruce, Austria.
Equivalent Strain Designations ATCC 10627, CBS 1890, CCRC 20468, IFO 0803, JCM 3590, NRRL Y-1482.
Reference Beck, Ann.Mycol. 20: 219-227, 1922.
Fuson et al., Int.J.Syst.Bacteriol. 29: 64-69, 1979 (DNA).
Yamada et al., J.Gen.Appl.Microbiol. 19: 189-208, 1973 (CoQ.)
Wickerham, L.J. 1951. taxonomy of yeasts. Technical Bulletin No. 1029, United States Dept. of Agriculture.
Synonomy of the yeast genera Hansenula and Pichia demonstrated through comparisons of DNA relatedness. Kurtzman, C.P., Antonie van Leeuwenhoek, 50, 209-217, 1984.
Pridham TG. New names and new combinations in the order Actinomycetales Buchanan 1917. U.S. Dep. Agric. Tech. Bull. 1424: 1-55, 1970.
Hou CT. pH dependence and thermostability of lipases from cultures from the ARS Culture Collection. J. Ind. Microbiol. 13: 242-248, 1994.

Physical Characteristics

Optimum Temperature Unknown
Miniumum Temperature Unknown
Maximum Temperature Unknown


Shape Elongated
Min Broth Breadth (µm) 3
Max Broth Breadth (µm) 4
Min Broth Length (µm) 5
Max Broth Length (µm) 39
Min Agar Breadth (µm) Unknown
Max Agar Breadth (µm) Unknown
Min Agar Length (µm) Unknown
Max Agar Length (µm) Unknown
Arrangement Chains
Colour on Agar Yellow
Surface on Agar Shiny
Texture on Agar Rough
Deposit in Broth Slimy
Ring in Broth Absent
Ring Colour Unknown
Pellicle in Broth Absent
Pellicle Appearance N/A
Pellicle Habit N/A

Cell Division

Budding Multipolar
Fission Absent

Filamentous Growth

Pseudomycelium Ill formed
Pseudomycelium Branch Unknown
Pseudomycelium Form Unknown
Blastospores Few
Blastospore Shape Oval
Blastospore Location Unknown
Blastospore Habit In chains
True Mycelium Present
Clamp Connections Absent

Asexual Spores

Ballistospores Absent
Arthrospores Absent
Endospores Absent
Chlamydospores Absent

Sexual Spores

Ascospores Absent
Ascospore Shape N/A
Ascospore Wall N/A
Ascospore No Per Ascus N/A
Ascus Shape N/A
Conjugation Absent
Teliospores Absent
Teliospore Shape N/A


Assay Unknown
Salt Tolerant Unknown
Killer Unknown
Plasmid Unknown

Semi-Anaerobic Fermentation

Glucose -
Galactose -
Sucrose -
Maltose -
Cellobiose -
Trehalose -
Lactose -
Melibiose -
Raffinose -
Melizitose -
Inulin -
Soluble Starch -
Xylose Unknown
A M D Glucoside -

Aerobic Utilisation and Growth - Sole Sources of Nitrogen

NH4 2SO4 Unknown
KNO3 +
Ethylamine Unknown
Cadaverine Unknown
Lysine Unknown


Vitamin Free Growth Unknown
Cyclohex 100ppm Unknown
Cyclohex 1000ppm Unknown
50% Glucose Growth Unknown
60% Glucose Growth Unknown
Lipolytic -
Acid Production Unknown
37c Growth Unknown
40c Growth Unknown
Arbutin Hydrolysis Weak/Latent
Urease Activity Unknown
Starch Production Unknown
Acid Tolerant Unknown