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NCYC 500

Lindnera mrakii

Pre 2011 Name Williopsis saturnus var. mrakii

Equivalent Strain Designations ATCC 10743, CBS 1707, CCRC 21416, DBVPG 6729, IFO 0897, JCM 3614, NRRL Y-1364, VKM Y-173.

Depositor L.J. Wickerham, NRRL.

Deposit Date April 1957

Habitat Soil, Dobodura area, Papua New Guinea.

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Lindnera mrakii

Aerobic Utilisation and Growth

Glucose +
Galactose -
Sorbose -
Sucrose -
Maltose -
Cellobiose +
Trehalose -
Lactose -
Melibiose Unknown
Raffinose Unknown
Melizitose -
Inulin -
Soluble Starch -
Xylose Unknown
L Arabinose -
D Arabinose Unknown
Ribose Unknown
Rhamnose Unknown
Ethanol Unknown
Glycerol Unknown
Erythritol -
Ribitol -
Galactitol Unknown
Mannitol Unknown
Sorbitol Unknown
A M D Glucoside Unknown
Salicin Unknown
Lactic Acid Unknown
Succinic Acid Unknown
Citric Acid Unknown
Inositol Unknown
Gluconolactone Unknown
Glucosamine Unknown
Methanol Unknown
Xylitol Unknown

Strain Information

Type Strain
Information Type strain of Hansenula mrakii.
Killer character K9Rj (Young), K9R9 (Wickner).
Depositor L.J. Wickerham, NRRL.
Deposit Name Hansenula mrakii, Williopsis mrakii
Month of deposit April
Deposit Year 1957
Habitat Soil, Dobodura area, Papua New Guinea.
Equivalent Strain Designations ATCC 10743, CBS 1707, CCRC 21416, DBVPG 6729, IFO 0897, JCM 3614, NRRL Y-1364, VKM Y-173.
Reference Wickerham, US Dep.Agric.Tech.Bull.No. 1029, Washington 1951, p.40.
Naumov et al., Dokl.Acad.Nauk.SSSR 259: 718-722, 1981.
Aono, System.Appl.Microbiol. 13: 394-397, 1990 (alkali tolerance)
Young, The Yeasts (eds Rose & Harrison), London: Academic Press, 1987.
Young & Yagiu, A.v.Leeuwenhoek 44: 59-77, 1978 (killer).
Tsuchiya et al., Jpn.J.Exp.Med. 28: 105-114, 1958 (serotype).
Shimizu et al. 1985. Killer properties of wine yeasts and characterisation of Killer wine yeasts. Journal of Fermentation technology, 63, 421-429.
Hodgson VJ, Button D, Walker GM. Anti-Candida activity of a novel killer toxin from the yeast Williopsis mrakii. Microbiology. 1995 Aug;141 ( Pt 8):2003-12

Physical Characteristics

Optimum Temperature Unknown
Miniumum Temperature Unknown
Maximum Temperature Unknown

Cells

Shape Oval
Min Broth Breadth (µm) 3
Max Broth Breadth (µm) 4
Min Broth Length (µm) 4
Max Broth Length (µm) 5
Min Agar Breadth (µm) 3
Max Agar Breadth (µm) 4
Min Agar Length (µm) 4
Max Agar Length (µm) 5
Arrangement Pairs
Colour on Agar Cream
Surface on Agar Dull
Texture on Agar Rough
Deposit in Broth Non-Flocculent
Ring in Broth Absent
Ring Colour Unknown
Pellicle in Broth Thin
Pellicle Appearance Wrinkled
Pellicle Habit Climbing

Cell Division

Budding Multipolar
Fission Absent

Filamentous Growth

Pseudomycelium Absent
Pseudomycelium Branch N/A
Pseudomycelium Form N/A
Blastospores N/A
Blastospore Shape N/A
Blastospore Location N/A
Blastospore Habit N/A
True Mycelium Absent
Clamp Connections Absent

Asexual Spores

Ballistospores Absent
Arthrospores Absent
Endospores Absent
Chlamydospores Absent

Sexual Spores

Ascospores Present
Ascospore Shape Saturn
Ascospore Wall Smooth
Ascospore No Per Ascus Unknown
Ascus Shape Unknown
Conjugation Absent
Teliospores Absent
Teliospore Shape N/A

Miscellaneous

Assay Unknown
Salt Tolerant Unknown
Killer Yes
Plasmid No

Semi-Anaerobic Fermentation

Glucose +
Galactose -
Sucrose -
Maltose -
Cellobiose Unknown
Trehalose Unknown
Lactose -
Melibiose -
Raffinose -
Melizitose Unknown
Inulin Unknown
Soluble Starch Unknown
Xylose Unknown
A M D Glucoside Unknown

Aerobic Utilisation and Growth - Sole Sources of Nitrogen

NH4 2SO4 Unknown
KNO3 +
Ethylamine Unknown
Cadaverine Unknown
Lysine Unknown

Other

Vitamin Free Growth +
Cyclohex 100ppm Unknown
Cyclohex 1000ppm Unknown
50% Glucose Growth Unknown
60% Glucose Growth Unknown
Lipolytic -
Acid Production Unknown
37c Growth Unknown
40c Growth Unknown
Arbutin Hydrolysis +
Urease Activity Unknown
Starch Production -
Acid Tolerant Unknown