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NCYC 175

Komagataella pastoris

Pre 2011 Name Pichia pastoris

Equivalent Strain Designations ATCC 28485, CCRC 21531, CBS 704, JCM 3650, NCTC 2058, NRRL Y-1603.

Depositor A. Guilliermond

Deposit Date Unknown 1925

Habitat Exudate of Chestnut tree, near Lyon, France.

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Komagataella pastoris

Aerobic Utilisation and Growth

Glucose +
Galactose -
Sorbose -
Sucrose -
Maltose -
Cellobiose -
Trehalose +
Lactose -
Melibiose -
Raffinose -
Melizitose -
Inulin -
Soluble Starch -
Xylose -
L Arabinose -
D Arabinose -
Ribose -
Rhamnose +
Ethanol +
Glycerol +
Erythritol -
Ribitol -
Galactitol -
Mannitol +
Sorbitol Weak/Latent
A M D Glucoside -
Salicin -
Lactic Acid Weak/Latent
Succinic Acid +
Citric Acid +
Inositol -
Gluconolactone -
Glucosamine -
Methanol +
Xylitol -

Strain Information

Type Strain
Information Production of yeast protein from hydrocarbons (US Patent 4,226,939).
Produces natural flavor aldehydes (Murray WD, 1989).
Converts ethanol to acetaldehyde (Duff SJ, Murray WD, 1988a; Duff SJ, Murray WD, 1988b; Duff SJ, Murray WD, 1988c; Appl. Microbiol. Biotechnol. 36: 82-86, 1991; Enzyme Microb. Technol. 11: 770-775, 1989).
Degrades ethyl alcohol (ethanol) (Duff SJ, Murray WD, 1988a; Duff SJ, Murray WD, 1988b; Duff SJ, Murray WD, 1988c; Appl. Microbiol. Biotechnol. 36: 82-86, 1991; Enzyme Microb. Technol. 11: 770-775, 1989).
Degrades hydrogen peroxide (Duff SJB, Murray WD, 1990).
Produces acetaldehyde (Duff SJ, Murray WD, 1988a; Duff SJ, Murray WD, 1988b; Duff SJ, Murray WD, 1988c; Appl. Microbiol. Biotechnol. 36: 82-86, 1991; Enzyme Microb. Technol. 11: 770-775, 1989).
Produces alcohol oxidase (Murray WD, et al, 1989; Murray WD, et al, 1990; Agric. Biol. Chem. 54: 1879-1882, 1990).
Depositor A. Guilliermond
Deposit Name Saccharomyces pastori / Zygosaccharomyces pastori
Month of deposit Unknown
Deposit Year 1925
Habitat Exudate of Chestnut tree, near Lyon, France.
Equivalent Strain Designations ATCC 28485, CCRC 21531, CBS 704, JCM 3650, NCTC 2058, NRRL Y-1603.
Reference Guilliermond, CR.Hebd. Séances Mem.Soc.Biol. 82: 466-470, 1919.
Nakase & Komagata, J.Gen.Appl.Microbiol. 16: 511-521, 1970 (GC).
Bicknell & Douglas, J.Bacteriol. 101: 505, 1970 (GC).
Yamada et al., J.Gen.Appl.Microbiol. 19: 189-208, 1973 (CoQ).
Duff SJ, Murray WD. Production and application of methylotrophic yeast Pichia pastoris. Biotechnol. Bioeng. 31: 44-49, 1988a.
Duff SJ, Murray WD. Comparison of free and immobilized Pichia pastoris cells for conversion of ethanol to acetaldehyde. Biotechnol. Bioeng. 31: 790-795, 1988b.
Duff SJ, Murray WD. Production of flavor aldehydes using nongrowing whole cells of Pichia pastoris. Ann. N.Y. Acad. Sci. 542: 428-433, 1988c.
Murray WD, et al. Induction and stability of alcohol oxidase in the methylotrophic yeast Pichia pastoris. Appl. Microbiol. Biotechnol. 32: 95-100, 1989.
Duff SJ, Murray WD. Oxidation of benzyl alcohol by whole cells of Pichia pastoris and by alcohol oxidase in aqueous and nonaqueous reaction media. Biotechnol. Bioeng. 34: 153-159, 1989.
Murray WD, et al. Catabolite inactivation in the methylotrophic yeast Pichia pastoris. Appl. Environ. Microbiol. 56: 2378-2383, 1990.
Wegner EH. Treatment of make-up water for use in a fermentation process for growth of yeast cells requiring growth factors. US Patent 4,226,939 dated Oct 7 1980
Murray WD, et al. Production of natural flavor aldehydes from natural source primary alcohols C2-C7. US Patent 4,871,669 dated Oct 3 1989
Phaff HJ. A proposal for amendment of the diagnosis of the genus Pichia Hansen. Antonie van Leeuwenhoek 22: 113-116, 1956.
Appl. Microbiol. Biotechnol. 36: 82-86, 1991.
Yamada Y, et al. The phylogenetic relationships of methanol-assimilating yeasts based on the partial sequences of 18S and 26S ribosomal RNAs: the proposal of Komagataella gen. nov. (Saccharomycetaceae). Biosci. Biotechnol. Biochem. 59: 439-444, 1995.
Enzyme Microb. Technol. 11: 770-775, 1989.
Agric. Biol. Chem. 54: 1879-1882, 1990.
Duff SJB, Murray WD. Metabolism of hydrogen peroxide by Pichia pastoris. Agric. Biol. Chem. 54: 1967-1974, 1990.

Physical Characteristics

Optimum Temperature Unknown
Miniumum Temperature Unknown
Maximum Temperature Unknown

Cells

Shape Oval
Min Broth Breadth (µm) 3
Max Broth Breadth (µm) 5
Min Broth Length (µm) 3
Max Broth Length (µm) 6
Min Agar Breadth (µm) 2
Max Agar Breadth (µm) 5
Min Agar Length (µm) 3
Max Agar Length (µm) 6
Arrangement Pairs
Colour on Agar Cream
Surface on Agar Shiny
Texture on Agar Smooth
Deposit in Broth Non-Flocculent
Ring in Broth Incomplete
Ring Colour White
Pellicle in Broth Absent
Pellicle Appearance N/A
Pellicle Habit N/A

Cell Division

Budding Multipolar
Fission Absent

Filamentous Growth

Pseudomycelium Well formed
Pseudomycelium Branch Irregularly branched
Pseudomycelium Form Mycocandida
Blastospores Absent
Blastospore Shape N/A
Blastospore Location N/A
Blastospore Habit N/A
True Mycelium Absent
Clamp Connections Absent

Asexual Spores

Ballistospores Absent
Arthrospores Absent
Endospores Absent
Chlamydospores Absent

Sexual Spores

Ascospores Present
Ascospore Shape Hat
Ascospore Wall Unknown
Ascospore No Per Ascus 4
Ascus Shape Unknown
Conjugation Present
Teliospores Absent
Teliospore Shape N/A

Miscellaneous

Assay Unknown
Salt Tolerant Unknown
Killer No
Plasmid No

Semi-Anaerobic Fermentation

Glucose +
Galactose -
Sucrose -
Maltose -
Cellobiose -
Trehalose -
Lactose -
Melibiose -
Raffinose -
Melizitose -
Inulin -
Soluble Starch -
Xylose Unknown
A M D Glucoside -

Aerobic Utilisation and Growth - Sole Sources of Nitrogen

NH4 2SO4 +
KNO3 -
Ethylamine +
Cadaverine +
Lysine Unknown

Other

Vitamin Free Growth Weak/Latent
Cyclohex 100ppm +
Cyclohex 1000ppm +
50% Glucose Growth -
60% Glucose Growth -
Lipolytic -
Acid Production Weak/Latent
37c Growth -
40c Growth Unknown
Arbutin Hydrolysis -
Urease Activity -
Starch Production -
Acid Tolerant Unknown