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NCYC 416

Kluyveromyces lactis

Pre 2011 Name Kluyveromyces lactis

Equivalent Strain Designations ATCC 56498, CBS 683, DBVPG 6305, DSM 4394, IFO 1090, NRRL Y-8279, UCD 71-59.

Depositor CBS

Deposit Date Unknown 1955

Habitat Gassy cheese.

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Kluyveromyces lactis

Aerobic Utilisation and Growth

Glucose +
Galactose +
Sorbose -
Sucrose +
Maltose +
Cellobiose +
Trehalose +
Lactose +
Melibiose -
Raffinose +
Melizitose +
Inulin -
Soluble Starch -
Xylose Weak/Latent
L Arabinose -
D Arabinose -
Ribose -
Rhamnose -
Ethanol +
Glycerol +
Erythritol -
Ribitol -
Galactitol -
Mannitol +
Sorbitol +
A M D Glucoside +
Salicin +
Lactic Acid +
Succinic Acid +
Citric Acid -
Inositol -
Gluconolactone -
Glucosamine Weak/Latent
Methanol -
Xylitol +

Strain Information

Type Strain
Information MT alpha.
Campbell serotype C.
Produces beta-galactosidase (beta-D-galactosidase) (Grieve PA, et al., 1983; Inchaurrondo VA, 1994).
Production of cheese-ripening proteinases (Grieve PA, et al., 1983).
Transformation host (Blondeau K, 1994; Appl. Microbiol. Biotechnol. 40: 306-308, 1993; Biotechnol. Tech. 7: 609-614, 1993).
Disinfectant testing.
Depositor CBS
Deposit Name Saccharomyces lactis
Month of deposit Unknown
Deposit Year 1955
Habitat Gassy cheese.
Equivalent Strain Designations ATCC 56498, CBS 683, DBVPG 6305, DSM 4394, IFO 1090, NRRL Y-8279, UCD 71-59.
Reference Allen & Thornley Ann.Appl.Biol. 16: 578, 1929.
Steensma, Curr.Genet. 14: 311-317.
GenLit: Coria et al, A.v.Leeuwenhoek 58: 227-234, 1990 (mtDNA).
Cong et al., Microbiology 140: 1327-1335, 1994 (plasmid).
Vaughan-Martini, A. & Martini, A. 1987. Taxonomic revision of the yeast genus Kluyveromyces by nuclear deoxyribonucleic acid reassociation. International Journal of Systematic Bacteriology, 37, 380-385.
Grieve PA, et al.. Partial characterization of cheese-ripening proteinases produced by the yeast Kluyveromyces lactis. J. Dairy Res. 50: 469-480, 1983.
Inchaurrondo VA, et al.. Yeast growth and -galactosidase production during aerobic batch cultures in lactose-limited synthetic medium. Process Biochem. 29: 47-54, 1994.
Blondeau K, et al. Physiological approach to heterologous human serum albumin production by Kluyveromyces lactis in chemostat culture. Yeast 10: 1297-1303, 1994.
Appl. Microbiol. Biotechnol. 40: 306-308, 1993.
Biotechnol. Tech. 7: 609-614, 1993.

Physical Characteristics

Optimum Temperature Unknown
Miniumum Temperature Unknown
Maximum Temperature Unknown

Cells

Shape Long-oval
Min Broth Breadth (µm) 2
Max Broth Breadth (µm) 4
Min Broth Length (µm) 3
Max Broth Length (µm) 7
Min Agar Breadth (µm) 5
Max Agar Breadth (µm) 7
Min Agar Length (µm) 6
Max Agar Length (µm) 8
Arrangement Unknown
Colour on Agar Cream
Surface on Agar Shiny
Texture on Agar Smooth
Deposit in Broth Flocculent
Ring in Broth Absent
Ring Colour Unknown
Pellicle in Broth Thin
Pellicle Appearance Milky
Pellicle Habit Climbing

Cell Division

Budding Multipolar
Fission Absent

Filamentous Growth

Pseudomycelium Well formed
Pseudomycelium Branch Irregularly branched
Pseudomycelium Form Mycocandida
Blastospores Absent
Blastospore Shape N/A
Blastospore Location N/A
Blastospore Habit N/A
True Mycelium Absent
Clamp Connections Absent

Asexual Spores

Ballistospores Absent
Arthrospores Absent
Endospores Absent
Chlamydospores Absent

Sexual Spores

Ascospores Absent
Ascospore Shape N/A
Ascospore Wall N/A
Ascospore No Per Ascus N/A
Ascus Shape N/A
Conjugation Absent
Teliospores Absent
Teliospore Shape Unknown

Miscellaneous

Assay No
Salt Tolerant Unknown
Killer Unknown
Plasmid No

Semi-Anaerobic Fermentation

Glucose +
Galactose Weak/Latent
Sucrose Weak/Latent
Maltose -
Cellobiose -
Trehalose -
Lactose Weak/Latent
Melibiose -
Raffinose Weak/Latent
Melizitose -
Inulin -
Soluble Starch -
Xylose Unknown
A M D Glucoside -

Aerobic Utilisation and Growth - Sole Sources of Nitrogen

NH4 2SO4 +
KNO3 -
Ethylamine +
Cadaverine +
Lysine Unknown

Other

Vitamin Free Growth -
Cyclohex 100ppm +
Cyclohex 1000ppm -
50% Glucose Growth -
60% Glucose Growth Unknown
Lipolytic -
Acid Production -
37c Growth -
40c Growth Unknown
Arbutin Hydrolysis +
Urease Activity -
Starch Production -
Acid Tolerant Unknown