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NCYC 240

Saccharomyces cerevisiae

Pre 2011 Name Saccharomyces cerevisiae

Equivalent Strain Designations Whitbread Taylor Walker Yeast SC12. ATCC 44953, MUCL 30938.

Depositor B.M. Brown

Deposit Date June 1951

Habitat Ale production yeast.

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Details

Saccharomyces cerevisiae

Aerobic Utilisation and Growth

Glucose +
Galactose +
Sorbose -
Sucrose +
Maltose +
Cellobiose -
Trehalose +
Lactose -
Melibiose -
Raffinose Weak/Latent
Melizitose Weak/Latent
Inulin -
Soluble Starch -
Xylose -
L Arabinose -
D Arabinose -
Ribose -
Rhamnose -
Ethanol Weak/Latent
Glycerol -
Erythritol -
Sorbitol -
Ribitol -
Galactitol -
Mannitol -
A M D Glucoside Weak/Latent
Salicin -
Lactic Acid -
Succinic Acid -
Citric Acid -
Inositol -
Gluconolactone -
Glucosamine -
Methanol -
Xylitol -

General

Pre 2011 Name Saccharomyces cerevisiae
Lodder Name N/A
In House Number N/A
Date Of Last Manipulation 26/5/2004

Brewing Information

Description N/A
Deposit 8-15mm
Head Formation Intermediate
Attenuation 1.010-1.012
Clarity of Final Beer 10-20
Fermentation Rate 8-10°
Flocculence N/A

Strain Information

Information Produces benzopyrene hydroxylase [benzo(a)pyrene hydroxylase]
O3, DMS 63 µg/l, high pyruvic, low acetic, S.G. 6.9, pH 3.91 used in BRF brewery where it has proved a stable yeast. Fermentation stops at 1012 in batch fermentation; no rising gas bubbles to keep it in suspension. Produces very rough variants which can form 20% of the population and do not assimilate maltotriose. Maltotriose is fermented relatively slowly by the normal strain. (See NCYC 753 & 754).
Depositor B.M. Brown
Deposit Name Saccharomyces cerevisiae
Month of deposit June
Deposit Year 1951
Habitat Ale production yeast.
Equivalent Strain Designations Whitbread Taylor Walker Yeast SC12. ATCC 44953, MUCL 30938.
Reference Woods LF , Wiseman A . Benzo(a) pyrene hydroxylase from Saccharomyces cerevisiae. Substance binding, spectral and kinetic data. Biochim. Biophys. Acta 613: 52-61, 1980.
Restriction analysis of 2µ plasmid, J.Inst.Brew., 1982, 88, 7-10.

Physical Characteristics

Optimum Temperature 30
Miniumum Temperature 6.6
Maximum Temperature 35

Cells

Shape Round-oval
Min Broth Breadth (µm) 5
Max Broth Breadth (µm) 8
Min Broth Length (µm) 8
Max Broth Length (µm) 10
Min Agar Breadth (µm) 4
Max Agar Breadth (µm) 6
Min Agar Length (µm) 6
Max Agar Length (µm) 10
Arrangement Pairs
Colour on Agar Cream
Surface on Agar Dull
Texture on Agar Smooth
Deposit in Broth Flocculent
Ring in Broth Absent
Ring Colour Unknown
Pellicle in Broth Absent
Pellicle Appearance N/A
Pellicle Habit N/A

Cell Division

Budding Multipolar
Fission Absent

Filamentous Growth

Pseudomycelium Absent
Pseudomycelium Branch N/A
Pseudomycelium Form N/A
Blastospores Absent
Blastospore Shape N/A
Blastospore Location N/A
Blastospore Habit N/A
True Mycelium Absent
Clamp Connections Absent

Asexual Spores

Ballistospores Absent
Arthrospores Absent
Endospores Absent
Chlamydospores Absent

Sexual Spores

Ascospores Absent
Ascospore Shape N/A
Ascospore Wall N/A
Ascospore No Per Ascus N/A
Ascus Shape N/A
Conjugation Absent
Teliospores Absent
Teliospore Shape N/A

Miscellaneous

Assay No
Salt Tolerant -
Killer No
Plasmid Yes

Semi-Anaerobic Fermentation

Glucose +
Galactose +
Sucrose +
Maltose +
Cellobiose -
Trehalose -
Lactose -
Melibiose -
Raffinose +
Melizitose +
Inulin -
Soluble Starch -
Xylose Unknown
A M D Glucoside +

Aerobic Utilisation and Growth - Sole Sources of Nitrogen

NH4 2SO4 +
KNO3 -
Ethylamine -
Cadaverine -
Lysine Unknown

Other

Vitamin Free Growth -
Cyclohex 100ppm -
Cyclohex 1000ppm -
50% Glucose Growth -
60% Glucose Growth -
Lipolytic -
Acid Production -
37c Growth +
40c Growth Unknown
Arbutin Hydrolysis -
Urease Activity -
Starch Production -
Acid Tolerant -