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NCYC 431

Saccharomyces cerevisiae

Pre 2011 Name Saccharomyces cerevisiae

Equivalent Strain Designations ATCC 44732, CBS 2358, NCYC 73, NRRL Y-132.

Depositor L.J. Wickerham

Deposit Date March 1955

Habitat Associated with fermentation

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Saccharomyces cerevisiae

Aerobic Utilisation and Growth

Glucose +
Galactose +
Sorbose -
Sucrose +
Maltose +
Cellobiose -
Trehalose Weak/Latent
Lactose -
Melibiose -
Raffinose Weak/Latent
Melizitose -
Inulin -
Soluble Starch -
Xylose -
L Arabinose -
D Arabinose -
Ribose -
Rhamnose -
Ethanol +
Glycerol -
Erythritol -
Sorbitol -
Ribitol -
Galactitol -
Mannitol -
A M D Glucoside -
Salicin -
Lactic Acid Weak/Latent
Succinic Acid -
Citric Acid -
Inositol -
Gluconolactone -
Glucosamine -
Methanol -
Xylitol -

General

Pre 2011 Name Saccharomyces cerevisiae
Lodder Name N/A
In House Number N/A
Date Of Last Manipulation 22/6/2004

Brewing Information

Description N/A
Deposit N/A
Head Formation N/A
Attenuation N/A
Clarity of Final Beer N/A
Fermentation Rate N/A
Flocculence N/A

Strain Information

Information Produces sterol-binding polysaccharides (Pillai CG , Weete JD , 1981).
Produces ethanol using membrane bioreactor (Cheryan M , Mehaia MA , 1984).
Transformation of organosulfides to sulfoxides (Biotechnol. Lett. 17: 1069-1074, 1995).
Produces ethyl alcohol [ethanol] (Cheryan M , Mehaia MA , 1984).
Produces polygalacturonase [pectinase] ( McKay AM, 1990).
Diploid, highly ethanol-tolerant (13% v/v)
Produces polysaccharides (Pillai CG , Weete JD, 1975).
Depositor L.J. Wickerham
Deposit Name Saccharomyces cerevisiae
Month of deposit March
Deposit Year 1955
Habitat Associated with fermentation
Equivalent Strain Designations ATCC 44732, CBS 2358, NCYC 73, NRRL Y-132.
Reference Ness F, et al. Identification of yeast strains using the polymerase chain reaction. J. Sci. Food Agric. 62: 89-94, 1993.
Biotechnol. Lett. 17: 1069-1074, 1995.
Pillai CG, Weete JD . Sterol-binding polysaccharides of Rhizopus arrhizus, Penicillium roquefortii and Saccharomyces carlsbergensis. Phytochemistry 14: 2347-2351, 1975.
Robinson CW, et al.. Ethanol production by immobilized yeast cells in a packed tower. Adv. Biotechnol. 2: 105-111, 1981.
Cheryan M, Mehaia MA . Ethanol production in a membrane recycle bioreactor. Process Biochem. 19: 204-208, 1984.
Hill GA , Robinson CW. Morphological behaviour of Saccharomyces cerevisiae during continuous fermentation. Biotechnol. Lett. 10: 815-820, 1988.
McKay AM. Degradation of polygalacturonic acid by Saccharomyces cerevisiae. Lett. Appl. Microbiol. 11: 41-44, 1990.
Ghase TK, Tyagi RD. Rapid ethanol fermentation of cellulose hydrolysate. II. Product and substrate inhibition and optimization of fermentor design. Biotechnol. Bioeng. 21: 1401-1420, 1979.
Pan et al., Anal.Chem. 25: 23, 1953 (analysis).

Physical Characteristics

Optimum Temperature 34
Miniumum Temperature 11
Maximum Temperature 39

Cells

Shape Round-oval
Min Broth Breadth (µm) 3
Max Broth Breadth (µm) 7
Min Broth Length (µm) 5
Max Broth Length (µm) 7
Min Agar Breadth (µm) 3
Max Agar Breadth (µm) 5
Min Agar Length (µm) 3
Max Agar Length (µm) 8
Arrangement Unknown
Colour on Agar Cream
Surface on Agar Shiny
Texture on Agar Smooth
Deposit in Broth Non-Flocculent
Ring in Broth Absent
Ring Colour Unknown
Pellicle in Broth Absent
Pellicle Appearance N/A
Pellicle Habit N/A

Cell Division

Budding Multipolar
Fission Absent

Filamentous Growth

Pseudomycelium Absent
Pseudomycelium Branch N/A
Pseudomycelium Form N/A
Blastospores Absent
Blastospore Shape N/A
Blastospore Location N/A
Blastospore Habit N/A
True Mycelium Absent
Clamp Connections Absent

Asexual Spores

Ballistospores Absent
Arthrospores Absent
Endospores Absent
Chlamydospores Absent

Sexual Spores

Ascospores Present
Ascospore Shape Round
Ascospore Wall Smooth
Ascospore No Per Ascus 4
Ascus Shape Unknown
Conjugation Absent
Teliospores Absent
Teliospore Shape Unknown

Miscellaneous

Assay No
Salt Tolerant Unknown
Killer Unknown
Plasmid Unknown

Semi-Anaerobic Fermentation

Glucose +
Galactose +
Sucrose +
Maltose +
Cellobiose -
Trehalose -
Lactose -
Melibiose -
Raffinose +
Melizitose -
Inulin -
Soluble Starch -
Xylose Unknown
A M D Glucoside -

Aerobic Utilisation and Growth - Sole Sources of Nitrogen

NH4 2SO4 +
KNO3 -
Ethylamine -
Cadaverine -
Lysine Unknown

Other

Vitamin Free Growth -
Cyclohex 100ppm -
Cyclohex 1000ppm Unknown
50% Glucose Growth -
60% Glucose Growth Unknown
Lipolytic -
Acid Production Weak/Latent
37c Growth +
40c Growth Unknown
Arbutin Hydrolysis -
Urease Activity -
Starch Production -
Acid Tolerant Unknown