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NCYC 87

Saccharomyces cerevisiae

Pre 2011 Name Saccharomyces cerevisiae

Equivalent Strain Designations ATCC 9763, CBS 2978, CCRC 20822, DSM 1333, NCTC 7239, NCTC 10716, NRRL Y-567.

Depositor ATCC

Deposit Date May 1947

Habitat Distillery yeast.

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Saccharomyces cerevisiae

Aerobic Utilisation and Growth

Glucose +
Galactose +
Sorbose -
Sucrose +
Maltose +
Cellobiose -
Trehalose +
Lactose -
Melibiose -
Raffinose +
Melizitose Weak/Latent
Inulin -
Soluble Starch -
Xylose -
L Arabinose -
D Arabinose -
Ribose -
Rhamnose -
Ethanol +
Glycerol -
Erythritol -
Sorbitol -
Ribitol -
Galactitol Unknown
Mannitol -
A M D Glucoside +
Salicin -
Lactic Acid Weak/Latent
Succinic Acid -
Citric Acid -
Inositol -
Gluconolactone -
Glucosamine -
Methanol -
Xylitol -

General

Pre 2011 Name Saccharomyces cerevisiae
Lodder Name SACCH.CEREVISIAE
In House Number N/A
Date Of Last Manipulation 11-2-2004

Brewing Information

Description N/A
Deposit N/A
Head Formation N/A
Attenuation N/A
Clarity of Final Beer N/A
Fermentation Rate N/A
Flocculence N/A

Strain Information

Information Applications:
Produces ethanol from saccharified whole corn mash (Ziffer J & Iosif MI , 1982).
Assay of amphotericin (British Pharmacopoeia Appendix XIV A, 2003).
Assay of amphotericin B (fungizone) (Pharmacopeia; USP USP28-NF23, 2005).
Assay of anisomycin (Bowman FW, 1957).
Assay of antifongine (Hagemann G, 1962).
Assay of candicidin (U.S. Pharmacopeia; USP USP28-NF23, 2005).
Assay of natamycin (pimaricin; tennecetin).
Assay of nystatin (fungicidin) (British Pharmacopoeia Appendix XIV A, 2003; European Pharmacopoeia EP 2.7.2, 1997; AOAC International. 957.23.; Bowman FW, 1957; Platt TB & Itkin AG, 1974).
Media testing (International Organization for Standardization/ANSI; ISO ISO 11133-2:2003).
Produces arginase; British Standard DD CEN ISO/TS 11133:2003).
Produces ethyl alcohol (ethanol) (Ziffer J & Iosif MI, 1982).
Produces histone deacetylase (Sanchez del Pino MM, 1994).
Produces nicotinic acid (niacin) (Tseng MM & Phillips CR, 1982).
Sterility testing (Bowman FW, 1971).
Susceptibility testing (Brenner DJ, 1994; Pfaller MA, 1994; Deutsches Institut fur Normung; DIN DIN 58940-84, 2002)
Testing disinfectants
Testing fungicides (British Standard BS EN 1650:1998; British Standard BS EN 12353:2006; British Standard BS EN 13697:2001; British Standard BS EN 14885:2006).
API Q C culture (bioMerieux Vitek) used in Commercial identification kits.
Depositor ATCC
Deposit Name Saccharomyces cerevisiae
Month of deposit May
Deposit Year 1947
Habitat Distillery yeast.
Equivalent Strain Designations ATCC 9763, CBS 2978, CCRC 20822, DSM 1333, NCTC 7239, NCTC 10716, NRRL Y-567.
Reference Ziffer J & Iosif MI. Temperature effects in ethanol fermentation high corn media. Biotechnol. Lett. 4: 809-814, 1982.
Microbial assay for antibiotics. Tokyo, Japan: Japanese Pharmacopoeia; JP JP14e.part I.34.
British Pharmacopoeia Commission. Biological assay of antibiotics. London, UK: British Pharmacopoeia Commission; British Pharmacopoeia Appendix XIV A, 2003.
U.S. Pharmacopeia. General Chapters; <81> ANTIBIOTICS-MICROBIAL ASSAYS. Rockville, MD: U.S. Pharmacopeia; USP USP28-NF23, 2005.
Bowman FW. Test organisms for antibiotic microbial assays. Antibiot. Chemother. 7: 639-640, 1957.
Hagemann G, et al.. Fungicidal compound and process of making same. US Patent 3,052,605 dated Sep 4 1962.
U.S. Pharmacopeia. General Chapters; <81> ANTIBIOTICS-MICROBIAL ASSAYS. Rockville, MD: U.S. Pharmacopeia; USP USP28-NF23, 2005.
British Pharmacopoeia Commission. Biological assay of antibiotics. London, UK: British Pharmacopoeia Commission; British Pharmacopoeia Appendix XIV A, 2003.
European Pharmacopoeia Commission. Microbiological assay of antibiotics. Strasbourg, France: European Pharmacopoeia Commission; European Pharmacopoeia EP 2.7.2, 1997.
AOAC International. Antibiotics in feeds, microbiological methods. Gaithersburg, MD: AOAC International; AOAC"Official Methods of Analysis of the AOAC International" 957.23.
Bowman FW . Test organisms for antibiotic microbial assays. Antibiot. Chemother. 7: 639-640, 1957.
Platt TB & Itkin AG . Microbiological assay of nystatin in feeds. J. Assoc. Off. Anal. Chem. 57: 536-540, 1974.
Microbiology of food and animal feeding stuffs-Guidelines on preparation and production of culture media- Part 2: Practical guidelines on performance testing of culture media. Geneva (Switzerland): International Organization for Standardization/ANSI; ISO ISO 11133-2:2003.
Microbiology of food and animal feeding stuffs - Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK: British Standards Institution; British Standard DD CEN ISO/TS 11133:2003.
Ziffer J & Iosif MI. Temperature effects in ethanol fermentation high corn media. Biotechnol. Lett. 4: 809-814, 1982.
Sanchez del Pino MM et al. Properties of the yeast nuclear histone deacetylase. Biochem. J. 303: 723-729, 1994.
Tseng MM & Phillips CR. The kinetics of yeast growth and nicotinic acid production. Biotechnol. Bioeng. 24: 1319-1325, 1982.
Bowman FW, et al. Collaborative study of aerobic media for sterility testing by membrane filtration. J. Pharm. Sci. 60: 1087-1088, 1971.
Brenner DJ , et al. Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates. J. Clin. Microbiol. 32: 1625-1628, 1994.
Pfaller MA , et al. Selection of candidate quality control isolates and tentative quality control ranges for in vitro susceptibility testing of yeast isolates by National Committee for Clinical Laboratory Standards proposed standard methods. J. Clin. Microbiol. 32: 1650-1653, 1994.
Medical microbiology-Susceptibility testing of microbial pathogens to antimicrobial agents - Part 84: Microdilution; Special requirements for testing of fungi against antifungal agents. Berlin, Germany: Deutsches Institut fur Normung; DIN DIN 58940-84: 2002, 2002.
Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of fungicidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas -- Test method and requirements (phase 2, step 1). London, UK: British Standards Institution; British Standard BS EN 1650:1998.
Chemical disinfectants and antiseptics - Preservation of test organisms used for the determination of bactericidal, sporicidal and fungicidal activity. London, UK: British Standards Institution; British Standard BS EN 12353:2006.
Chemical disinfectants and antiseptics - Quantitative non-porous surface test for the evaluation of bactericidal and/or fungicidal activity of chemical disinfectants used in food, industrial, domestic and institutional areas --- Test method and requirements without mechanical action (phase 2/step 2). London, UK: British Standards Institution; British Standard BS EN 13697:2001.
Chemical disinfectants and antiseptics --- Application of European Standards for chemical disinfectants and antiseptics. London, UK: British Standards Institution; British Standard BS EN 14885:2006.
US Fed.Regulations 27: 13047 & 1322, 1962 and 28: 3823, 1963.
Clemons et al., J.Infect.Dis. 169: 859-867, 1994.
McCusker, Infect.Immun. 62: 5447-5455, 1994.
British Pharmacopoeia, 1980, 2, A123.
US Pharmacopoeia, 1984, 20, %th suppl., 1098-1102.
J. Biol. Chem., 1980, 255, 6387 (studies on invertase).


Physical Characteristics

Optimum Temperature Unknown
Miniumum Temperature Unknown
Maximum Temperature Unknown

Cells

Shape Round-oval
Min Broth Breadth (µm) 4
Max Broth Breadth (µm) 7
Min Broth Length (µm) 4
Max Broth Length (µm) 12
Min Agar Breadth (µm) 3
Max Agar Breadth (µm) 9
Min Agar Length (µm) 5
Max Agar Length (µm) 13
Arrangement Single
Colour on Agar Cream
Surface on Agar Dull
Texture on Agar Wrinkled
Deposit in Broth Non-Flocculent
Ring in Broth Complete
Ring Colour White
Pellicle in Broth Thick
Pellicle Appearance Islands
Pellicle Habit Climbing

Cell Division

Budding Multipolar
Fission Absent

Filamentous Growth

Pseudomycelium Well formed
Pseudomycelium Branch Unknown
Pseudomycelium Form Unknown
Blastospores Absent
Blastospore Shape Unknown
Blastospore Location Unknown
Blastospore Habit Unknown
True Mycelium Absent
Clamp Connections Absent

Asexual Spores

Ballistospores Absent
Arthrospores Absent
Endospores Absent
Chlamydospores Absent

Sexual Spores

Ascospores Present
Ascospore Shape Round
Ascospore Wall Smooth
Ascospore No Per Ascus 2-4
Ascus Shape Unknown
Conjugation Absent
Teliospores Absent
Teliospore Shape Unknown

Miscellaneous

Assay Yes
Salt Tolerant -
Killer Unknown
Plasmid Yes

Semi-Anaerobic Fermentation

Glucose +
Galactose +
Sucrose +
Maltose +
Cellobiose -
Trehalose -
Lactose -
Melibiose -
Raffinose -
Melizitose -
Inulin -
Soluble Starch Unknown
Xylose Unknown
A M D Glucoside -

Aerobic Utilisation and Growth - Sole Sources of Nitrogen

NH4 2SO4 +
KNO3 -
Ethylamine -
Cadaverine -
Lysine Unknown

Other

Vitamin Free Growth -
Cyclohex 100ppm -
Cyclohex 1000ppm -
50% Glucose Growth -
60% Glucose Growth -
Lipolytic -
Acid Production Weak/Latent
37c Growth +
40c Growth Unknown
Arbutin Hydrolysis -
Urease Activity -
Starch Production -
Acid Tolerant -