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NCYC 55

Pichia kudriavzevii

Pre 2011 Name Issatchenkia orientalis

Equivalent Strain Designations NCTC 483

Depositor A. Klocker, Carlsberg laboratories, Copenhagen.

Deposit Date April 1920

Habitat Fly (Calliphora erythrocephala) found in garden near Carlsberg. Isolated 1900.

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Pichia kudriavzevii

Aerobic Utilisation and Growth

Glucose +
Galactose -
Sorbose -
Sucrose -
Maltose -
Cellobiose -
Trehalose -
Lactose -
Melibiose -
Raffinose -
Melizitose -
Inulin -
Soluble Starch -
Xylose -
L Arabinose -
D Arabinose -
Ribose -
Rhamnose -
Ethanol +
Glycerol +
Erythritol -
Ribitol -
Galactitol -
Mannitol -
Sorbitol -
A M D Glucoside -
Salicin -
Lactic Acid Weak/Latent
Succinic Acid +
Citric Acid -
Inositol -
Gluconolactone -
Glucosamine -
Methanol -
Xylitol -

Strain Information

Information No
Depositor A. Klocker, Carlsberg laboratories, Copenhagen.
Deposit Name Pichia membranaefaciens var. calliphorae
Month of deposit April
Deposit Year 1920
Habitat Fly (Calliphora erythrocephala) found in garden near Carlsberg. Isolated 1900.
Equivalent Strain Designations NCTC 483
Reference C.R. Lab. Carlsberg, 1911-17, 10, 211.
A.E.Wiles J.I.B. 1950. p192.

Physical Characteristics

Optimum Temperature Unknown
Miniumum Temperature Unknown
Maximum Temperature Unknown

Cells

Shape Long-oval
Min Broth Breadth (µm) 2
Max Broth Breadth (µm) 4
Min Broth Length (µm) 5
Max Broth Length (µm) 14
Min Agar Breadth (µm) 3
Max Agar Breadth (µm) 4
Min Agar Length (µm) 9
Max Agar Length (µm) 13
Arrangement Pairs
Colour on Agar Yellow
Surface on Agar Dull
Texture on Agar Wrinkled
Deposit in Broth Flocculent
Ring in Broth Absent
Ring Colour Unknown
Pellicle in Broth Thin
Pellicle Appearance Milky
Pellicle Habit Climbing

Cell Division

Budding Multipolar
Fission Absent

Filamentous Growth

Pseudomycelium Well formed
Pseudomycelium Branch Regularly branched
Pseudomycelium Form No
Blastospores Absent
Blastospore Shape No
Blastospore Location No
Blastospore Habit No
True Mycelium Absent
Clamp Connections Absent

Asexual Spores

Ballistospores Absent
Arthrospores Absent
Endospores Absent
Chlamydospores Absent

Sexual Spores

Ascospores Present
Ascospore Shape Hat
Ascospore Wall Smooth
Ascospore No Per Ascus 2
Ascus Shape Oval
Conjugation Absent
Teliospores Absent
Teliospore Shape No

Miscellaneous

Assay No
Salt Tolerant 10%
Killer No
Plasmid Unknown

Semi-Anaerobic Fermentation

Glucose -
Galactose -
Sucrose -
Maltose -
Cellobiose -
Trehalose -
Lactose -
Melibiose -
Raffinose -
Melizitose -
Inulin -
Soluble Starch -
Xylose Unknown
A M D Glucoside -

Aerobic Utilisation and Growth - Sole Sources of Nitrogen

NH4 2SO4 +
KNO3 -
Ethylamine +
Cadaverine +
Lysine Unknown

Other

Vitamin Free Growth +
Cyclohex 100ppm -
Cyclohex 1000ppm -
50% Glucose Growth -
60% Glucose Growth -
Lipolytic -
Acid Production -
37c Growth -
40c Growth Unknown
Arbutin Hydrolysis -
Urease Activity -
Starch Production -
Acid Tolerant -